Tumor-associated macrophages impair NK cell IFN-γ production and contribute to tumor progression in clear cell renal cell carcinoma.

Tumor-associated macrophages (TAM) are abundant in several tumor types and usually correlate with poor prognosis. Previously, we demonstrated that anti-inflammatory macrophages (M2) inhibit NK cell effector functions. Here, we explored the impact of TAM on NK cells in the context of clear-cell renal cell carcinoma (ccRCC). Bioinformatics analysis revealed that an exhausted NK cell signature strongly correlated with an M2 signature. Analysis of TAM from human ccRCC samples confirmed that they exhibited an M2-skewed phenotype and inhibited IFN-γ production by NK cells. Moreover, human M0 macrophages cultured with conditioned media from ccRCC cell lines generated macrophages with an M2-skewed phenotype (TAM-like), which alike TAM, displayed suppressive activity on NK cells. Moreover, TAM depletion in the mouse Renca ccRCC model resulted in delayed tumor growth and reduced volume, accompanied by an increased frequency of IFN-γ-producing tumor-infiltrating NK cells that displayed heightened expression of T-bet and NKG2D and reduced expression of the exhaustion-associated co-inhibitory molecules PD-1 and TIM-3. Therefore, in ccRCC, the tumor microenvironment polarizes TAM toward an immunosuppressive profile that promotes tumor-infiltrating NK cell dysfunction, contributing to tumor progression. In addition, immunotherapy strategies targeting TAM may result in NK cell reinvigoration, thereby counteracting tumor progression.

Stent Syndrome: Does the Diameter Matter? Results of a Randomized Trial.

Introduction: Double-J (DJ) ureteral stents are used for multiple purposes in urology. Even though they temporize the subsequent treatment of lithiasis, they may cause different symptoms that impact quality of life (QoL). Purpose: In this randomized trial, we assessed whether the diameter of ureteral stents has an impact on catheter-associated symptoms, and their impact on QoL. Methods: A total of 194 consecutive patients undergoing DJ insertion between December 2018 and December 2022 were prospectively enrolled and divided into three categories: 4.7F (Group 1, n = 71), 6F (Group 2, n = 65), and 7F (Group 3, n = 58). Within 1 week after the DJ placement, patients completed the validated Spanish version of the Ureteral Stent Symptoms Questionnaire. Continuous variables were compared using analysis of variance and Student's t-tests. For categorical data, the chi-square test was used. Results: In the domain of "work" and "additional problems," there were significant differences. In the "Work" domain, Group 1 presented the lower symptoms. In the domain "additional problems," patients in Group 1 were prescribed fewer antibiotics owing to low urinary tract symptoms. In question U4 about urinary incontinence, patients in Groups 2 and 3 developed these symptoms more than patients in Group 1. In the "sexual activity" domain, specifically in question S3 (the patient has ever suffered any type of pain during sexual activity?), patients with 4.7F presented lower scores than patients with larger catheters. Conclusion: DJ-related symptoms affect QoL in most cases. Smaller catheters produced significantly less urinary incontinence, faster work reincorporation, fewer symptoms related to sexual activity, and fewer catheter-related symptoms than 7F catheters. In contrast, Group 3 presented fewer outpatient visits because of symptoms related to the DJ.

Dusting efficacy between the regular setting of holmium laser (Ho:YAG) versus Vapor Tunnel pulse modality for non-complex kidney stones.

The new pulse modality Vapor-Tunnel™ (VT) consists of a very long pulse that uses the minimum peak power, causing the energy to pass through a previously created vapor channel or tunnel. The first part of the pulse creates a vapor channel, whereas the remaining energy is discharged immediately after, passing straight through the previously created tunnel. The aim of this study is to compare the dusting efficacy between Ho:YAG laser with long pulse and Ho:YAG laser with VT for non-complex kidney stones. A retrospective comparative study of 236 patients who underwent retrograde intrarenal surgery using Ho:YAG laser (long pulse vs. VT) was performed. Stone size, stone density, laser settings, laser emission time, and total operative time were recorded. We also assessed the lithotripsy efficacy (J/mm3). The stone-free rate was defined as the absence of stone fragments in a non-contrast abdominal computed tomography 4 weeks after the procedure. A total of 118 patients were included in each group. There was no significant difference in age, gender, and body mass index. Median stone volume (737 mm3 vs. 636 mm3) and stone density (788 HU vs. 656 HU) were higher in the VT group. Total energy used (14.5 J vs. 18.2 J), the laser emission time (20 min vs. 26 min), and the total operative time (79.5 min vs. 95 min) were significantly lower in the VT group. The stone-free rate was comparable between both groups (74.5% for VT and 66.1% for the long-pulse group, p = 0.15). When we evaluated the efficacy of laser lithotripsy, a significantly lower difference was obtained in the VT group (median 12.5 J/mm3 vs. median 23.1 J/mm3). The VT pulse modality was associated with decreased laser time and operative time. Additionally, it increased lithotripsy efficacy compared to Ho:YAG long pulse laser, but with a comparable free-stone rate.

Validation of an instrument to evaluate the satisfaction of the professional practice in pertaining students to the program of Nutrition and Dietary / Validación de un instrumento para evaluar la satisfacción de la práctica profesional en estudiantes pertenecientes a la carrera de Nutrición y Dietética Introducción

Professional practice is a relevant process for the personal and academic development of students. It uses learning contexts located in real scenarios and enables the acquisition of knowledge, skills, and competencies necessary for professional practice. Objective. To design and validate an instrument designed to evaluate students' satisfaction with theirprofessional practice process. Materials and methods. Mixed-method study, non-probabilistic sample by convenience of 196 participants. The sample is formed by 20 Nutritionists and 176 students belonging to the Nutrition and Dietetics undergraduate program. The statistical software SPSS version 22 was used to analyze reliability by Cronbach's alpha and the Delphi method for content validity. An instrument with 32 items grouped in 4 dimensions was elaborated and applied to 116 students to analyze reliability. The instrument was adjusted to 25 items, and content validity was analyzed by a panel of experts. The judge'sconsensus was the foundation for developing a 29-item version grouped into 4 dimensions. This version was applied to 60 students to obtainCronbach's alpha validation. Results. The instrument presents an excellent level of reliability, with a Cronbach's Alpha coefficient of 0.927. Conclusions. The instrument designed to measure satisfaction with professional practices is a reliable and valid measure, since it allows knowing the students 'evaluation at the end of their professional practice process(AU)

La práctica profesional es un proceso relevante para el desarrollo personal y académico de los estudiantes, utiliza contextos de aprendizaje situados en escenarios reales, posibilita la adquisición de conocimientos, habilidades y competencias necesarias para el ejercicio profesional. Objetivo. Diseñar y validar un instrumento, que permita evaluar la satisfacción de los estudiantes sobre su proceso de práctica profesional. Materiales y métodos. Estudio de tipo mixto, muestra no probabilística por conveniencia de 196 participantes, distribuidos en 20 Nutricionistas y 176 estudiantes pertenecientes a la carrera de Nutrición y Dietética. Sé utilizó el software estadístico SPSS versión 22, para analizar la confiabilidad por Alfa de Cronbach y el método Delphi para la validez del contenido. Se elaboró un instrumento con 32 ítems agrupados en 4 dimensiones, se aplicó a 116 estudiantes para analizar la confiabilidad. Se ajustó el instrumento a 25 ítems, se analizó la validez del contenido por panel de expertos. El consenso de los jueces dio origen a una versión, con 29 ítems agrupados en 4 dimensiones, se aplicó finalmente a 60 estudiantes para obtener el Alfa de Cronbach. Resultados. El instrumento presenta un nivel excelente de confiabilidad, con un coeficiente de Alfa de Cronbach de 0.927. Conclusiones. El instrumento diseñado para medir la satisfacción de las prácticas profesionales, es una medida confiable y válida, ya que permite conocer la valoración de los estudiantes al finalizar su proceso de práctica profesional(AU)

Effectiveness and Safety of Thulium Fiber Laser in the Conservative Management of Patients with Upper Tract Urothelial Carcinoma.

Background: Few clinical data are available on thulium fiber laser (TFL) and conservative treatment of upper urinary tract urothelial carcinoma (UTUC). Objective: To assess the effectiveness and safety of TFL in the conservative treatment of UTUC in terms of both tumor ablation and complication rates in a short-term follow-up. Design setting and participants: Retrospective data were collected from all patients who underwent endoscopic management of UTUC between January 2021 and April 2022. All patients with nonmetastatic UTUC who were deemed suitable candidates (low- and high-grade disease) for conservative treatment were reviewed. Intervention: All patients underwent ureteroscopy with biopsy and at 2, 6, and 12 mo after the first surgery. UTUC ablation was achieved using TFL. Outcome measurements and statistical analysis: Clinical data were collected in a dedicated database. Intra- and postoperative outcomes were assessed. A descriptive statistical analysis was performed. Results and limitations: In total, 28 patients were evaluated. Thirteen patients (46.4%) were included in the low-risk UTUC treatment group and 15 (53.6%) in the high-risk group. The mean tumor size was 15.3 ± 5.7 mm. Biopsy showed low- and high-grade UTUCs in 19 and eight patients, respectively. Only one biopsy was inconclusive for achieving a diagnosis. At the second procedure biopsy, no tumor was found in 19 cases (70.4%), whereas seven had tumors confirmed (25.9%). To date, 23 and 17 out of 26 patients completed the 6- and 12-mo follow-up, respectively. UTUC recurrence was detected in five of 23 patients (21.7%) and in three of 17 patients (17.7%) at 6 and 12 mo, respectively. A total of 95 procedures were performed. No intraoperative complications were observed. In ten of the 95 procedures (10.5%), Clavien-Dindo grade I-II postoperative complications were experienced. Only one grade IIIB postoperative complication was noted. Conclusions: TFL is a safe and effective technique for conservative treatment of UTUC in a short-term follow-up. Optimal tumor ablation and fine hemostatic control were achieved without major complications. Patient summary: In this study, we looked at the outcomes of upper urinary tract tumors conservatively treated with the new thulium fiber laser (TFL). We conclude that TFL represents a safe and effective technique for the treatment of this kind of tumors in a short-term follow-up.

The MICA-NKG2D axis in clear cell renal cell carcinoma bolsters MICA as target in immuno-oncology.

NKG2D is a major natural killer (NK) cell-activating receptor that recognizes eight ligands (NKG2DLs), including MICA, and whose engagement triggers NK cell effector functions. As NKG2DLs are upregulated on tumor cells but tumors can subvert the NKG2D-NKG2DL axis, NKG2DLs constitute attractive targets for antibody (Ab)-based immuno-oncology therapies. However, such approaches require a deep characterization of NKG2DLs and NKG2D cell surface expression on primary tumor and immune cells. Here, using a bioinformatic analysis, we observed that MICA is overexpressed in renal cell carcinoma (RCC), and we also detected an association between the NKG2D-MICA axis and a diminished overall survival of RCC patients. Also, by flow cytometry (FC), we observed that MICA was the only NKG2DL over-expressed on clear cell renal cell carcinoma (ccRCC) tumor cells, including cancer stem cells (CSC) that also coexpressed NKG2D. Moreover, tumor-infiltrating leukocytes (TIL), but not peripheral blood lymphoid cells (PBL) from ccRCC patients, over-expressed MICA, ULBP3 and ULBP4. In addition, NKG2D was downregulated on peripheral blood NK cells (PBNK) from ccRCC patients but upregulated on tumor-infiltrating NK cells (TINK). These TINK exhibited impaired degranulation that negatively correlated with NKG2D expression, diminished IFN-γ production, upregulation of TIM-3, and an impaired glucose intake upon stimulation with cytokines, indicating that they are dysfunctional, display features of exhaustion and an altered metabolic fitness. We conclude that ccRCC patients exhibit a distorted MICA-NKG2D axis, and MICA emerges as the forefront NKG2DL for the development of targeted therapies in ccRCC.

Simultaneous Bilateral Endoscopic Surgery (SBES): Is It Ready for Prime Time?

Introduction: To date, some data available in literature on simultaneous bilateral endoscopic surgery (SBES) have shown good outcomes in terms of both effectiveness and safety. The aim of this study was to report the outcomes pertaining to the effectiveness and safety of SBES performed in our series of patients with bilateral renal stones. Materials and Methods: A prospective analysis of consecutive patients who underwent SBES for bilateral renal stones at our institution between June 2017 and September 2021 was performed. Routine preoperative and 1-month postoperative work-up included history, physical examination, urinalysis, urine culture, and blood tests, including the evaluation of estimated glomerular filtration rate using the Cockcroft-Gault equation. An abdominal noncontrast CT scan was performed in all cases preoperatively and 1 month postoperatively. Peri-/postoperative complications were reported according to the Clavien-Dindo classification system. The primary endpoint of the study was stone-free rate (SFR), and the secondary endpoints were Clavien-Dindo complications grade 1 or higher. Results: Altogether, 101 patients met the inclusion criteria and were enrolled in the study. SFR for all renal units was achieved in 82 patients (81.1%) at the 1-month follow-up. Twelve patients underwent additional flexible ureteroscopy for residual fragments, and 7 asymptomatic patients with single small residual fragment were observed. Eighteen patients (17.8%) experienced Clavien-Dindo Grade I-II complications, whereas one patient (1%) experienced Clavien-Dindo Grade IIIa complication (renal arteriovenous fistula embolization under local anesthesia). Conclusions: SBES is a safe and effective procedure for the treatment of bilateral renal stones. Further randomized studies with larger populations are needed to confirm these favorable outcomes of SBES to establish it as an alternative to staged surgeries in patients with bilateral renal stones. Appropriate patient selection is of paramount importance for achieving good outcomes without experiencing severe complications.

[Moderate/severe hydronephrosis before ureteral double-j stent insertion is associated with less back pain during micturition.] / La hidronefrosis moderada/severa antes de la inserción del catéter ureteral doble J se asocia con menos dolor de espalda durante la micción.

OBJECTIVE: Symptoms related toureteral double-J stent are the most common complaintamong patients. Back pain during micturition isone of the most frequent but intensity and durationmay vary between them. This study aimed to assessthe relationship between hydronephrosis grade beforeureteral double-j stent insertion and back pain duringmicturition. PATIENTS AND METHODS: All consecutive patientsundergoing a ureteral double-j insertion secondaryto urolithiasis between 2017 and 2019 were prospectivelyenrolled. Hydronephrosis was assessed using aCT scan and was categorized according to renal pelvismeasure as 0-10 mm (low group), and ≥10 mm(moderate group). At one week postoperative, patientsymptoms were evaluated using the Spanish validatedversion of the Ureteral Stent Symptom Questionnaire(USSQ) and 10 cm visual analog scale (VAS). Meanscores for USSQ domains and mean VAS scores werecompared. RESULTS: 149 patients were included and evaluated.The mean age was 48.2±15.1 years. We found no significantdifferences in mean scores for each main domainin the USSQ or VAS. The presence of flank painduring micturition was present in 73.5% and 52.5 %for the low vs moderate hydronephrosis group respectively(p=0.01). Sub-analysis by gender was 78.7 % vs44.0 % (p=0.00) and 58.6% vs 66.6% (p=0.6) of low andmoderate group in men and women respectively.Analysis of the USSQ item score revealed thatthe moderate hydronephrosis group presented less''pain or discomfort when urinating,'' less ''presenceof back pain when urinating,'' less ''admission orprocedure because of discomfort,'' and less ''feelingabout reinsertion''. CONCLUSION: Patients in the moderate hydronephrosisgroup did not have significant advantagesin terms of voiding symptoms, pain, general health,sexual matters, or additional problems determined bythe USSQ or in VAS. However, sub-analysis reportedadvantages concerning pain and additional questions.Men with moderate hydronephrosis presented statisticallysignificantly less pain during micturition.

INTRODUCCIÓN Y OBJETIVO: Lossíntomas relacionados con el catéter ureteral doble-Json la queja más común en los pacientes. El dolor deespalda durante la micción es uno de los más frecuentes,pero la intensidad y la duración varían entre ellos.El objetivo de este estudio fue evaluar la relación entreel grado de hidronefrosis antes de la inserción del catéterureteral doble-J y el dolor de espalda durante lamicción. PACIENTES Y MÉTODOS: Se incluyeron prospectivamentetodos los pacientes consecutivos que sesometieron a la inserción de un catéter ureteral doblej debido a la urolitiasis entre 2017 y 2019. La hidronefrosisse evaluó mediante una tomografía computarizaday se clasificó según la medida de la pelvis renalcomo 0-10 mm (grupo bajo), y ≥10 mm (grupo moderado/severo). Una semana después de la operación,los síntomas del paciente se evaluaron utilizando laversión validada en español del Cuestionario de Síntomasdel Catéter Ureteral (USSQ) y la la escala visualanáloga (EVA) de 10 cm. Se compararon las puntuaciones medias de los dominios del USSQ y las puntuacionesmedias de la EVA. RESULTADOS: Se incluyeron y evaluaron 149 pacientes.La edad media fue de 48,2±15,1 años. No seencontraron diferencias significativas en las puntuacionesmedias de cada dominio principal en la USSQo la EVA. La presencia de dolor en el flanco durante lamicción estuvo presente en el 73,5% y el 52,5% parael grupo de hidronefrosis baja vs. moderada respectivamente(p=0,01). El subanálisis por género fue 78,7% vs 44,0 % (p=0,00) y 58,6 % vs 66,6 % (p=0,6) delgrupo de baja y moderada en hombres y mujeres respectivamente.El análisis de la puntuación de los ítems de laUSSQ reveló que el grupo de hidronefrosis moderadapresentó menos "dolor o molestia al orinar", menos"presencia de dolor de espalda al orinar", menos "admisióno procedimiento por molestia" y menos "sensaciónde reinserción". CONCLUSIÓN: Los pacientes del grupo de hidronefrosismoderada no ofrecen ventajas significativas encuanto a los síntomas de evacuación, el dolor, la saludgeneral, las cuestiones sexuales o los problemasdeterminados por el USSQ o en EVA. Sin embargo, elsubanálisis informó de ventajas relativas al dolor ypreguntas adicionales. Los hombres con hidronefrosismoderada presentaban un dolor estadísticamente significativodurante la micción.

La hidronefrosis moderada/severa antes de la inserción del catéter ureteral doble j se asocia con menos dolor de espalda durante la micción / Moderate/severe hydronephrosis before ureteral double-j stent insertion is associated with less back pain during micturition

INTRODUCCIÓN Y OBJETIVO: Lossíntomas relacionados con el catéter ureteral doble-Json la queja más común en los pacientes. El dolor deespalda durante la micción es uno de los más frecuentes, pero la intensidad y la duración varían entre ellos.El objetivo de este estudio fue evaluar la relación entreel grado de hidronefrosis antes de la inserción del catéter ureteral doble-J y el dolor de espalda durante lamicción.PACIENTES Y MÉTODOS: Se incluyeron prospectivamente todos los pacientes consecutivos que sesometieron a la inserción de un catéter ureteral doblej debido a la urolitiasis entre 2017 y 2019. La hidronefrosis se evaluó mediante una tomografía computarizada y se clasificó según la medida de la pelvis renalcomo 0-10 mm (grupo bajo), y ≥10 mm (grupo moderado/severo). Una semana después de la operación,los síntomas del paciente se evaluaron utilizando laversión validada en español del Cuestionario de Síntomas del Catéter Ureteral (USSQ) y la la escala visualanáloga (EVA) de 10 cm. Se compararon las puntuaciones medias de los dominios del USSQ y las puntuaciones medias de la EVA.RESULTADOS: Se incluyeron y evaluaron 149 pacientes. La edad media fue de 48,2±15,1 años. No seencontraron diferencias significativas en las puntuaciones medias de cada dominio principal en la USSQo la EVA. La presencia de dolor en el flanco durante lamicción estuvo presente en el 73,5% y el 52,5% parael grupo de hidronefrosis baja vs. moderada respectivamente (p=0,01). El subanálisis por género fue 78,7% vs 44,0 % (p=0,00) y 58,6 % vs 66,6 % (p=0,6) delgrupo de baja y moderada en hombres y mujeres respectivamente.El análisis de la puntuación de los ítems de laUSSQ reveló que el grupo de hidronefrosis moderadapresentó menos “dolor o molestia al orinar”, menos“presencia de dolor de espalda al orinar”, menos “admisión o procedimiento por molestia” y menos “sensación de reinserción”.

OBJECTIVE: Symptoms related toureteral double-J stent are the most common complaint among patients. Back pain during micturition isone of the most frequent but intensity and durationmay vary between them. This study aimed to assessthe relationship between hydronephrosis grade beforeureteral double-j stent insertion and back pain duringmicturition.PATIENTS AND METHODS: All consecutive patientsundergoing a ureteral double-j insertion secondaryto urolithiasis between 2017 and 2019 were prospectively enrolled. Hydronephrosis was assessed using aCT scan and was categorized according to renal pelvis measure as 0-10 mm (low group), and ≥10 mm(moderate group). At one week postoperative, patientsymptoms were evaluated using the Spanish validatedversion of the Ureteral Stent Symptom Questionnaire (USSQ) and 10 cm visual analog scale (VAS). Meanscores for USSQ domains and mean VAS scores werecompared.RESULTS: 149 patients were included and evaluated.The mean age was 48.2±15.1 years. We found no significant differences in mean scores for each main domain in the USSQ or VAS. The presence of flank painduring micturition was present in 73.5% and 52.5 %for the low vs moderate hydronephrosis group respectively (p=0.01). Sub-analysis by gender was 78.7 % vs44.0 % (p=0.00) and 58.6% vs 66.6% (p=0.6) of low andmoderate group in men and women respectively.Analysis of the USSQ item score revealed thatthe moderate hydronephrosis group presented less‘‘pain or discomfort when urinating,’’ less ‘‘presence of back pain when urinating,’’ less ‘‘admission orprocedure because of discomfort,’’ and less ‘‘feelingabout reinsertion’’.CONCLUSION: Patients in the moderate hydronephrosis group did not have significant advantagesin terms of voiding symptoms, pain, general health,sexual matters, or additional problem

Tumor-Experienced Human NK Cells Express High Levels of PD-L1 and Inhibit CD8+ T Cell Proliferation.

Natural Killer (NK) cells play a key role in cancer immunosurveillance. However, NK cells from cancer patients display an altered phenotype and impaired effector functions. In addition, evidence of a regulatory role for NK cells is emerging in diverse models of viral infection, transplantation, and autoimmunity. Here, we analyzed clear cell renal cell carcinoma (ccRCC) datasets from The Cancer Genome Atlas (TCGA) and observed that a higher expression of NK cell signature genes is associated with reduced survival. Analysis of fresh tumor samples from ccRCC patients unraveled the presence of a high frequency of tumor-infiltrating PD-L1+ NK cells, suggesting that these NK cells might exhibit immunoregulatory functions. In vitro, PD-L1 expression was induced on NK cells from healthy donors (HD) upon direct tumor cell recognition through NKG2D and was further up-regulated by monocyte-derived IL-18. Moreover, in vitro generated PD-L1hi NK cells displayed an activated phenotype and enhanced effector functions compared to PD-L1- NK cells, but simultaneously, they directly inhibited CD8+ T cell proliferation in a PD-L1-dependent manner. Our results suggest that tumors might drive the development of PD-L1-expressing NK cells that acquire immunoregulatory functions in humans. Hence, rational manipulation of these regulatory cells emerges as a possibility that may lead to improved anti-tumor immunity in cancer patients.

Construction of a 3D Surgical Model for Minimally Invasive Partial Nephrectomy: the Urotrainer VK-1.

PURPOSE OF REVIEW: Minimally invasive partial nephrectomy (MIPN) is a very challenging technique and complications may occur during its learning curve. It is important to acquire surgical skills to minimize operative risks. Surgical skills may be acquired through simulation devices or mentoring by a mentor. RECENT FINDINGS: We designed a 3D operable model with multiple and variant surgical scenarios in order to facilitate surgical training in MIPN. The model delivered a real-life feel and handle. To our knowledge, this is the first minimally invasive partial nephrectomy simulator to encompass a series of scenarios with multiple levels of difficulty while delivering a real-life experience.

Minimally Invasive Partial Nephrectomy: Are All Clamps Created Equal? An Ex Vivo Model Simulation of Vascular Clampage.

REASON FOR REVIEW: During the partial nephrectomy, clamping of the vascular pedicle before exision of the tumor is a key step in minimizing blood loss and maintaining adequate visualization. Different vascular clamping devices have been developed for minimal invasive surgery. However, there are no reports comparing them in turn of efficiency RECENT FINDINGS: We present an ex vivo experimental model, designed to demonstrate differences between the clamping devices. All clamps proved to function properly without any leakage at 90 and 120 mmHg, respectively. Our study and the ex vivo model prove that all available clamps are equally efficient at physiologic pressures.

Minimally invasive management of Zinner's syndrome / Manejo mínimamente invasivo del síndrome de Zinner

Abstract Zinner's syndrome (ZS) is a rare congenital malformation of the seminal vesicles and ipsilateral upper urinary tract which is characterized by the triad of ipsilateral ejaculatory duct obstruction, semi nal vesicle cysts, and renal agenesis. We report an 18-year-old male who presented intermittent gross hematuria and hematospermia and ejaculation volume diminution. ZS was diagnosed with magnetic resonance imaging (MRI) of the prostate, among other complementary studies requested. We performed a minimally laparoscopic resection of the left seminal vesicle. The postoperative was uneventful symptoms resolved, and 3 months later semen analysis showed increase in ejaculation volume. Currently, the minimally invasive approach is feasible and effective for the treatment of the seminal vesicle cysts excision in the ZS. We presented a symptomatic patient treated by a laparoscopic approach with successful middle follow-up results.

Resumen El síndrome de Zinner (ZS) es una malformación congénita rara de las vesículas seminales y del tracto urinario superior ipsolateral que se caracteriza por la tríada de obstrucción del conducto eyaculatorio ipsolateral, quistes de vesículas seminales y agenesia renal. Presentamos el caso de un varón de 18 años que presentó hematuria macroscópica intermitente y hemospermia y disminución del volumen de eyaculación. El diagnóstico de ZS se realizó mediante resonan cia magnética (RM) de próstata, entre otros estudios complementarios solicitados. Realizamos una resección mínimamente laparoscópica de la vesícula seminal izquierda. El postoperatorio se resolvió sin complicaciones y 3 meses después el análisis de semen mostró un aumento en el volumen de eyaculación. Actualmente, el abordaje mínimamente invasivo es factible y efectivo para el tratamiento de la escisión de quistes de vesículas seminales en el ZS. Presentamos un paciente sintomático tratado por vía laparoscópica con seguimiento medio exitoso.

[Active surveillance for testicular tumors: adherence and safety.] / Adherencia y seguridad de la vigilancia activa en tumores de testículo.

INTRODUCTION: The active surveillance (AS) of testicular tumors (seminoma and non-seminoma) is the most frequent management option in the stage I disease. Relapses generally occurred within the first 3 years and <5% appear after this time cut-off point is fulfilled. Therefore, the adherence is one of the most important pillars in the AS protocol. The aim of this study is to evaluate the adherence to the AS protocol in a community hospital and, in turn, evaluate the safety of it emphasizing in the relapse-free rate in patients with and without risk factors. MATERIALS AND METHODS: A retrospective study of all the patients included in the AS protocol with seminoma tumors (ST) or non-seminoma tumors (NST) stage I was performed. Postoperative controls were performed according to the NCCN (National Comprehensive Cancer Network) recommendations. Different variables were taken into account, emphasizing in the risk factors: testicular tumor >4cm and the rete testis invasion in the ST, the linfovascular invasion and the percentage>40% of embrionary carcinoma in the NST. Adherence to the AS protocol was evaluated, focusing on those patients who lost it and what time it occurred. RESULTS: A total of 64 patients were included. The median follow-up was 36 months (IC 21-48 months). 12 patients lost the follow-up during the protocol with a median follow-up of 27.5 months (IC 16-30 months). A 21.8% of patients entered in the AS protocol with some associated risk factor. Adherence follow-up was successful in the first year (96.8%) and decreased over time (92.2% at 24 months and 86.3% at 36 months). CONCLUSION: We presented an important adherence to the AS protocol in patients with clinical stage I testicular cancer and in our series there no recurrences after 36 months of follow-up.

INTRODUCCIÓN: La vigilancia activa (VA) de tumores testiculares seminoma (TS) y no-seminomas (TNS) es en la actualidad, la opción de manejo más frecuente utilizada en tumores testiculares estadio clínico I. Las recaídas dentro de este seguimiento se presentan generalmente dentro de los 3 años y <5% se presentan después de este periodo. La adherencia en la VA termina siendo un pilar fundamental.OBJETIVO: El objetivo de este trabajo es evaluar la adherencia al protocolo de vigilancia activa, y a su vez evaluar la seguridad de esta opción de manejo haciendo hincapié en la tasa libre de recaída en pacientes con y sin factores de riesgo.MATERIALES Y MÉTODOS: Se realizó un estudio retrospectivo de todos los pacientes incluidos en un protocolo de VA (TS y TNS estadio I). Se tomaron en cuenta diferentes variables, realizando hincapié en la evaluación de los diferentes factores de riesgo, tomando como tal en TS al tamaño testicular > de 4 cm y a la invasión de la rete testis en el resultado anatomopatológico. En cuanto a los TNS, la ILV y un porcentaje >40% de CE fueron los factores de riesgo evaluados. Se evaluó la adherencia al seguimiento del protocolo de VA, haciendofoco en aquellos pacientes que se perdieron del mismo y en qué momento ocurrió.RESULTADOS: Un total de 64 pacientes fueron incluidos a protocolo de VA. La mediana de seguimiento fue de 36 meses (IC 21-48 meses). De todos los pacientes incluidos en este estudio, 12 de ellos perdieron el seguimiento durante el esquema propuesto, presentando una mediana de seguimiento de 27,5 meses (IC 16-30 meses). Un 21,8% de pacientes ingresó al protocolo de VA con algún factor de riesgo asociado. La adherencia al seguimiento fue exitosa en el primer año con un porcentaje de adhesión que alcanzó el 96,8% y fue descendiendo con el paso del tiempo (92,2% a los 24 meses y 86,3% a los 36 meses).CONCLUSIÓN: En nuestra serie, se evidenció una marcada adhesión al protocolo de VA en pacientes con diagnóstico de tumor testicular estadio clínico I, sin registrar recurrencias después de los 36 meses de seguimiento.

Adherencia y seguridad de la vigilancia activa en tumores de testículo / Adherence and safety of active surveillance in testicular tumors

Introducción: La vigilancia activa(VA) de tumores testiculares seminoma (TS) y no-seminomas (TNS) es en la actualidad, la opción de manejomás frecuente utilizada en tumores testiculares estadioclínico I. Las recaídas dentro de este seguimiento sepresentan generalmente dentro de los 3 años y <5% sepresentan después de este periodo. La adherencia en laVA termina siendo un pilar fundamental.Objetivo: El objetivo de este trabajo es evaluar la adherencia al protocolo de vigilancia activa, y a su vezevaluar la seguridad de esta opción de manejo haciendo hincapié en la tasa libre de recaída en pacientescon y sin factores de riesgo. Materiales y métodos: Se realizó un estudio retrospectivo de todos los pacientes incluidos en un protocolode VA (TS y TNS estadio I). Se tomaron en cuenta diferentes variables, realizando hincapié en la evaluaciónde los diferentes factores de riesgo, tomando como talen TS al tamaño testicular > de 4 cm y a la invasiónde la rete testis en el resultado anatomopatológico. Encuanto a los TNS, la ILV y un porcentaje >40% de CEfueron los factores de riesgo evaluados. Se evaluó laadherencia al seguimiento del protocolo de VA, haciendo foco en aquellos pacientes que se perdieron del mismo y en qué momento ocurrió.Resultados: Un total de 64 pacientes fueron incluidosa protocolo de VA. La mediana de seguimiento fue de36 meses (IC 21-48 meses). De todos los pacientesincluidos en este estudio, 12 de ellos perdieron el seguimiento durante el esquema propuesto, presentandouna mediana de seguimiento de 27,5 meses (IC 16-30meses). Un 21,8% de pacientes ingresó al protocolo deVA con algún factor de riesgo asociado.La adherencia al seguimiento fue exitosa en el primeraño con un porcentaje de adhesión que alcanzó el96,8% y fue descendiendo con el paso del tiempo(92,2% a los 24 meses y 86,3% a los 36 meses).Conclusión: En nuestra serie, se evidenció una marcada adhesión al protocolo de VA en pacientes con...(AU)

Introduction: The active surveillance(AS) of testicular tumors (seminoma and non-seminoma)is the most frequent management option in the stageI disease. Relapses generally occurred within the first3 years and <5% appear after this time cut-off pointis fulfilled. Therefore, the adherence is one of the mostimportant pillars in the AS protocol. The aim of this studyis to evaluate the adherence to the AS protocol in acommunity hospital and, in turn, evaluate the safety of itemphasizing in the relapsefree rate in patients with andwithout risk factors.Materials and methods: A retrospective study ofall the patients included in the AS protocol with seminoma tumors (ST) or non-seminoma tumors (NST) stage Iwas performed. Postoperative controls were performedaccording to the NCCN (National ComprehensiveCancer Network) recommendations. Different variableswere taken into account, emphasizing in the risk factors:testicular tumor >4cm and the rete testis invasion in theST, the linfovascular invasion and the percentage>40%of embrionary carcinoma in the NST. Adherence to theAS protocol was evaluated, focusing on those patientswho lost it and what time it occurred.Results: A total of 64 patients were included. Themedian follow-up was 36 months (IC 21-48 months).12 patients lost the follow-up during the protocol with amedian follow-up of 27.5 months (IC 16-30 months). A21.8% of patients entered in the AS protocol with someassociated risk factor.Adherence follow-up was successful in the first year(96.8%) and decreased over time (92.2% at 24 monthsand 86.3% at 36 months).Conclusion: We presented an important adherence to the AS protocol in patients with clinical stage Itesticular cancer and in our series there no recurrencesafter 36 months of follow-up.(AU)

PI-RADS 3 lesions: Does the association of the lesion volume with the prostate-specific antigen density matter in the diagnosis of clinically significant prostate cancer?

INTRODUCTION: Currently, a new subclassification of the Pi-RADS 3 lesions and subgroups is being used: 3a (indolent or low-risk lesions with volume <0.5 ml) and 3b (significant or high-risk lesions with volume ≥0.5 ml). The prostate-specific antigen density (PSAd) has been identified as a diagnostic tool that helps to predict clinically significant prostate cancer (csCaP). The aim of this study is to evaluate the association of the volume of the Pi-RADS 3 lesions and the PSAd in the diagnosis of csCaP. MATERIAL AND METHODS: We conducted a retrospective study that included prostate biopsies performed using a transperineal approach and guided by ultrasound between 2015 and 2020. csCaP was defined as Gleason score ≥3 + 4. The population was divided into groups according to the Pi-RADS 3 subclassification and the PSAd value. We calculated sensitivity, specificity, negative predictive value (NPV) and positive predictive value (PPV) of 3b lesions for the detection of high-grade prostate cancer, alone and combined with PSAD groups. RESULTS: In total, 99 patients with Pi-RADS 3 lesions were included. Forty-three patients were in group 3a and 56, in 3b. Mean PSA was 7.28 ± 2.6 ng/ml. Pi-RADS 3a lesion did not present csCaP but 17.8% of Pi-RADS 3b lesion did. In group 3b with PSAd > 0.15, 62.5% presented csCaP. In those Pi-RADS 3b with PSAd ≤ 0.15, all biopsies were insignificant prostate cancer (isCaP) and 40 biopsies could have been avoided. Considering 3b as positive for csCaP detection, sensitivity was 100%, specificity 48.3%, NPV 17.8%, and PPV 100%. When adding PSAd to group 3b, sensitivity was 100%, specificity was 86.9%, NPV was 62.5%, PPV was 100%. In total, only the subgroup 3b with PSAd > 0.15 presented csCaP and 83.8% biopsies could be avoided. CONCLUSIONS: In this series, the association of the volume of PIRADS 3 lesion and the PSAd improves specificity and PPV contributing to improve the management of csCaP.

[Urinary continence prediction after laparoscopic radical prostatectomy according to preoperative urethral length measurement in multiparametric prostate resonance.] / Predicción de la continencia urinaria post ­ prostatectomía radical laparoscópica utilizando la medición de la longitud uretral en la resonancia multiparamétrica de próstata.

OBJECTIVE: Urinary incontinence (UI) is one of the main complications of radical prostatectomy. Multiple studies have attempted to find factors that may predict early recovery of urinary continence in surgically treated patients. The aim of this study is to evaluate the ability of the length of the membranous urethra (LUM) and the intraprostatic urethra (IUL) measured in preoperative by multiparametric prostate resonance imaging (MRI), to predict post-radical prostatectomy urinary continence. MATERIAL AND METHODS: A retrospective study between 2016 and 2018 was conducted. Patients who under went laparoscopic radical prostatectomy (LRP) and had a previous RMI were included. Different variables were evaluated: age, PSA, BMI, clinical and pathological stage, and the validated questionnaire of urinary incontinence (ICIQ-SF) was performed at 12 months postoperatively. LUM and LUIP were calculated and compared groups (continence vs incontinence) using the Student test (t). A value of p<0.05  was taken as significant. A ROC (Receiver Operating Characteristic) curve was made to predict continence according to LUM and LUIP. RESULTS: Forty-four patients were included. Mean age was 63 years (SD±7.2) and mean PSA was 9.2 ng/dL (SD±5.2). Seventy-five percent presented clinical stageT1c and 43.2% pathological stage pT2cN0. 20.5% (9 patients) presented some degree of UI. The mean LUM was 15.1 mm (SD±5.6) and LUIP 26 mm (SD±10.4). For patients with continence and incontinence, the mean LUM was 18.3 vs 9.5 mm (p=0.0001) and LUIP 31.5vs 15 mm (p=0.0001) respectively. The area under the curve taking as a value of LUM >10mm was 88% and LUIP>20mm was 86.7%. CONCLUSION: The use of preoperative mp RMN measurement of urethral membrane length and intraprostatic urethra may be useful in significantly predicting post-PR urinary continence.

OBJETIVO: La incontinencia de orina (IO) es una de las principales complicaciones de la prostatectomía radical. Múltiples estudios han tratado de encontrar factores para predecir la recuperación temprana de la continencia urinaria en pacientes tratados quirúrgicamente. El objetivo de este trabajo es evaluar la capacidad de la longitud de la uretra membranosa (LUM) y de la uretra intraprostática (LUIP) medida en la resonancia multiparamétrica de próstata (mpRMN) preoperatoria, para predecir la continencia urinaria post-prostatectomía radical. MATERIALES Y MÉTODOS: Se realizó un estudio retrospectivo, entre el año 2016 y 2018. Se incluyeron pacientes que fueron sometidos a una prostatectomía radical laparoscópica (PRL) y presentaban una mpRMN previa. Se evaluaron diferentes variables: edad, PSA, IMC, estadio clínico y patológico, y se realizó el cuestionario validado de incontinencia urinaria (ICIQ-SF) a los 12 meses postoperatorios. Se calcularon LUM y LUIP medidas por mpRMN y compararon grupos (continencia vs incontinencia) utilizando el Student test (t). Se tomó un valor de p<0,05 como estadísticametne significativo. Se realizó una curva COR (Característica Operativa del Receptor) para predecir continencia según LUM y LUIP. RESULTADOS: Se incluyeron 44 pacientes. La edad media fue 63 años (DE±7,2) y la media de PSA fue de 9,2 ng/dl (DE±5,2). El 75% presentaron estadio clínico T1c y un 43,2% estadio patológico pT2cN0. La media de LUM fue de 15,1 mm (DE±5,6) y de LUIP 26 mm (DE±10,4). El 20,5% (9 pacientes) presentaba algún grado de IO. Para pacientes con continencia e incontinencia las medias de LUM 18,3 vs 9,5 mm (p=0,0001) y LUIP de 31,5 vs 15 mm (p=0,0001) respectivamente. El área bajo la curva tomando como un valor de LUM >10mm fue del 88% y LUIP >20mm fue de 86,7%. CONCLUSIÓN: La utilización de la medición de la longitud de la uretra membranosa y de la uretra intraprostática por mpRMN preoperatorias, podrían ser útiles para predecir la continencia urinaria post-PR.